Services provided

 
 

Fresh Chilled


Fresh chilled semen refers to the preparation of semen and cooling to refrigeration temperatures (5-8’C) so that it can be shipped overnight for insemination within 24 hours.  The sperm rich fraction of the semen is collected and a nutrient/buffer is added to it.  It is then placed in a shipping container which will keep it at refrigeration temperature for up to 12 hours.  On arrival it is placed into the bitch either vaginally, surgically or via TCI.


The semen is thought to survive 72 hours after collection using this method.  There is also the possibility of freezing the chilled semen once it arrives at the receiving veterinarian.  However this would require that the extenders used in the chilling process are compatible with the freezing buffers used at the receiving facility.

Frozen Semen


The initial steps in semen freezing are similar to the preparation of semen for fresh chilled shipping in that semen quality is assessed and if adequate,  a nutrient is added and the semen is cooled to refrigeration temperature to 2 or more hours.  At this time the motility is checked and if still adequate, a glycerol containing solution is added to the semen.  This protects the sperm from the freezing process.

Fresh Chilled and Frozen Semen


VetCor Canine Reproduction Services

19337 - 48 Avenue, Surrey, BC  Canada  V3S-0L5  Tel: (604)372-0334 Fax: (604)372-3779 

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Straw in Goblet Attached to Cane

Shipper and Storage Container



Services Provided:

  1. Ovulation Timing

  2. Semen Evaluation

  3. TCI

  4. Fresh Chilled And Frozen Semen


Infertility Information Pages:

  1. Bitch

  2. Stud Dog


Because the semen is thought to live 72 hours with this method it is felt that conception rates may be higher  because the ovulation timing is not as critical and there is a greater number of sperm available at the time the egg is ready for fertilization.

The semen is then generally loaded into straws which are individually labeled with the stud dog’s information. These straws are then suspended over liquid nitrogen vapour for a short period of time before they are plunged into the liquid nitrogen.  The straws are then loaded into goblets which are attached to aluminum canes and placed in liquid nitrogen containing storage tanks.


Because the semen must remain frozen at all times until just before use, the shipping of frozen semen requires a very specialized container called a dry shipper.  This shipper will keep semen frozen for 7-14 days while underway.  However due to the value and size of the container, shipping is significantly more expensive than the shipping of fresh chilled semen.  Once thawed, the semen is only viable 12-24 hours and must be placed directly in the uterus at the exact time the eggs are ready for fertilization.